FGF9 BioAssay(TM) ELISA Kit (Porcine) (Fibroblast Growth Factor-9)

Fibroblast growth factor-9(FGF-9) is a steroid-regulated mitogen and survival factor for nerve and mesenchymal cells. The Porcine FGF-9 cDNA cloned by using oligonucleotide probes encodes a polypeptide consisting of 208aa. Sequence similarity to other members of the FGF family has been estimated to be around 30%. FGF-9 is an autocrine estromedin endometrial stromal growth factor that plays roles in cyclic proliferation of uterine endometrial stroma. FGF9 is produced and secreted by the prostatic stromal cells. It is a potent mitogen for both prostatic epithelial and stromal cells in culture. FGF9 is an abundant secreted growth factor that can act as both a paracrine mitogen for epithelial cells and an autocrine mitogen for stromal cells. Overexpression of this paracrine and autocrine growth factor may play an important role in the epithelial and stromal proliferation in benign prostatic hyperplasia. As a result of glycosylation, the molecular mass is 25-27kD. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Porcine FGF9. Sensitivity: <15pg/ml, Range: 62.5pg/ml-4000pg/ml, Specificity: Recognizes natural and recombinant Porcine FGF9. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Porcine FGF9 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Porcine FGF9 has been precoated onto 96-well plates. Standards(Expression system for standard: sf21, Immunogen sequence: M1-S208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Porcine FGF9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine FGF9 amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Porcine FGF9 antibody, 1x plate, Lyophilized recombinant Porcine FGF9 standard, 2x10ng/tube, Biotinylated anti-Porcine FGF9 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.