FGF1 (Heparin-binding Growth Factor 1) BioAssay(TM) ELISA Kit (Monkey)

Heparin-binding growth factor 1 is a protein that in humans is encoded by the FGF1 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. The FGF1 gene was mapped to chromosome 5q31.3-q33.2 by in situ hybridization. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA). Intended Use: Sandwich High Sensitivity BioAssay(TM) ELISA kit for Quantitative Detection of Monkey FGF1. Sensitivity: <10pg/ml, Range: 31.2pg/ml-2000pg/ml, Specificity: Recognizes natural and recombinant Monkey FGF1. There is no detectable cross-reactivity with other relevant proteins. Test Principle: This Monkey FGF1/Fgf Acidic BioAssay(TM) ELISA Kit was based on standard sandwich enzyme- linked immune-sorbent assay technology. A specific for Monkey primate FGF1/Fgf Acidic has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: A2-D155) and test samples are added to the wells, a biotinylated detection specific for Monkey primate FGF1/Fgf Acidic is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Monkey FGF1/Fgf Acidic amount of sample captured in plate. Kit Components: 96-well plate precoated with anti-Monkey FGF1 antibody, 1x plate, Lyophilized recombinant Monkey FGF1 standard, 2x10ng/tube, Biotinylated anti-Monkey FGF1 antibody, 1x130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100), Sample diluent buffer, 1x30ml, Antibody diluent buffer, 1x12ml, ABC diluent buffer, 1x12ml, TMB color developing agent, 1x10ml, TMB stop solution, 1x10ml, PBS washing buffer Powder for 1000ml, 1x buffer, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.