DMP-1, BioAssay(TM) ELISA Kit (Mouse) (Dentin Matrix Acidic Phosphorprotein 1)

Dentin matrix acidic phosphorprotein 1 is a protein that in humans is encoded by the DMP1 gene. It belongs to the small integrin-binding ligand N-linked glycoprotein(SIBLING) family of secreted phosphoproteins. This gene is mapped to 4q22.1. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. In undifferentiated osteoblasts, DMP-1 is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation, the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates, Intended Use: For quantitative detection of mouse DMP-1 in cell culture supernates, serum, plasma (heparin, EDTA) and tissue homogenates. Sensitivity: <10pg/ml, Range: 156-10,000pg/ml, Specificity: Natural and recombinant mouse DMP-1, Test Principle: This mouse DMP-1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for DMP-1 has been precoated onto 96-well plates. Standards(NSO, L17-Y503) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for DMP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse DMP-1 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse DMP-1 antibody, 1, Lyophilized recombinant mouse DMP-1 standard, 10ng/tube×2, Biotinylated mouse DMP-1 antibody, 130ul (dilution 1:100), Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100), Sample diluent buffer, 30ml, Antibody diluent buffer, 12ml, ABC diluent buffer, 12ml, TMB color developing agent, 10ml, TMB stop solution, 10ml, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.