17-phenyl trinor Prostaglandin F2alpha ELISA Kit

17-phenyl trinor Prostaglandin F2alpha (17-phenyl trinor PGF2alpha) is a metabolically stable analog of PGF2alpha and is a potent agonist for the FP receptor. It binds to the FP receptor on ovine luteal cells with a relative potency of 756% compared to that of PGF2alpha. At the rat recombinant FP receptor expressed in CHO cells, 17-phenyl trinor PGF2alpha inhibits PGF2alpha binding with a Ki value of 1.1 nM. In human and animal models of glaucoma, FP receptor agonist activity corresponds very closely with intraocular hypotensive activity. 17-phenyl trinor PGF2alpha ethyl amide is an F-series prostaglandin analog which has been approved for use as an ocular hypotensive drug, sold under the Allergan trade name Bimatoprost. Whether 17-phenyl trinor PGF2alpha ethyl amide is a prodrug analogous to prostaglandin ester prodrugs such as latanoprost is currently controversial. There is some evidence that unmetabolized 17-phenyl trinor PGF2alpha is a weak FP receptor agonist. 17-phenyl trinor PGF2alpha is converted by an amidase enzymatic activity in the bovine and human cornea to yield the corresponding free acid, with a conversion rate of about 40 µg/g corneal tissue/24 hours. Cayman's 17-phenyl trinor PGF2alpha ELISA is a sensitive detection method for measuring both the free acid and ethyl amide forms of 17-phenyl trinor PGF2alpha. The assay is most appropriate for use when only one of the two forms is present. Samples containing mixtures of both the ethyl amide and free acid should be purified and the two compounds separated prior to ELISA analysis.Synonyms: Bimatoprost (free acid), 17-phenyl trinor Prostaglandin F2alpha EIA Kit. Assay Range: 1.56-200 pg/ml. Sensitivity: 80% B/B0: 2.6 pg/ml. Mid-point: 50% B/B0: 15 pg/ml. Cross Reactivity: (17-phenyl trinor Prostaglandin F2alpha (free acid)) 100%, (17-phenyl trinor Prostaglandin F2alpha ethyl amide) 80%, (Latanoprost (free acid)) 1.50%, (Latanoprost) 0.90%, (5,6-trans Latanoprost) 0.40%, (Fluprostenol) <0.01%, (Fluprostenol isopropyl ester) <0.01%, (2,3-dinor Fluprostenol) <0.01%, (9-keto Fluprostenol) <0.01%, (11-keto Fluprostenol) <0.01%, (15-epi Latanoprost) <0.01%, (tetranor-PGEM) <0.01%, (tetranor-PGFM) <0.01%, (Prostaglandin D2) <0.01%, (Prostaglandin E2) <0.01%, (Prostaglandin E2 Ethanolamide) <0.01%, (6-keto Prostaglandin F1alpha) <0.01%, (Prostaglandin F2alpha) <0.01%, (Prostaglandin F2alpha Ethanolamide) <0.01%, (5-trans Prostaglandin F2alpha) <0.01%, (Thromboxane B2) <0.01%, (Unoprostone) <0.01%, (Unoprostone isopropyl ester) <0.01%. Origin: Animal/Bovine, Animal/Eel, Animal/Rabbit, Animal/Mouse.