SUV39H2, Chemiluminescent, BioAssay(TM) Kit (KMT1B, Suppressor Of Variegation 3-9 Homolog 2 (Drosoph

Intended Use:, The SUV39H2 Direct Activity BioAssay(TM) Kit is designed to measure SUV39H2 activity for profiling and screening applications, using purified SUV39H2. Test Principle: The SUV39H2 Direct Activity BioAssay(TM) Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H2 enzyme for 100 enzyme reactions. The key to the SUV39H2 Activity BioAssay(TM) Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate is added to produce chemiluminescence that can then be measured using a chemiluminescence reader. Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt, Applications: Great for studying enzyme kinetics, screening small molecular inhibitors for drug discovery and HTS applications. Kit Components: Plate precoated with histone substrate, 1x96 wells, SUV39H2, 1x4ug, 100uM S-adenosylmethionine 1x250ul , Primary antibody 1, 1x100ul , Secondary HRP-labeled antibody 1, 1x10ul, 4x HMT Assay Buffer 1, 1x3ml , Blocking buffer, 1x50ml , HRP chemiluminescent substrate, 2 components x 6ml each, Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.