DNMT1 direct activity assay kit

The DNMT1 Direct Activity Assay kit is designed to measure DNMT1 activity using purified enzyme complex or DNMT1 extracts. The DNMT1 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate pre-coated with DNMT substrate, the antibody against 5-methylcytosine, the secondary Europium-labeled antibody, S-adenosylmethionine, DNMT1 assay buffer, and purified DNMT1 for 100 enzyme reactions. The key to the DNMT1 Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine residue of the substrate. With this kit, only three simple steps on a microtiter plate are required for DNMT1 detection. First, S- adenosylmethionine is incubated with a sample containing assay buffer and DNMT1 for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.