DNA Polymerase beta Assay Kit

The DNA Polymerase beta Assay Kit is a fluorogenic assay designed to measure DNA Polymerase beta activity for screening and profiling applications, using AccuGreen(TM) as detection reagent. The kit comes in a convenient 96-well format, with enough recombinant Polymerase beta enzyme, DR Substrate 3, dNTP mix and buffer for 100 enzymatic reactions. Note: AccuGreen(TM) High Sensitivity dsDNA Quantitation Kit is sold separately by Biotium. DNA Polymerase beta uses available nucleotides to synthesize a DNA strand complementary to the template strand, leading to the formation of double-stranded DNA molecule (dsDNA). The amount of dsDNA formed directly correlates to the polymerase activity and can quantified by the addition of the fluorescent DNA-binding dye which allows to distinguish dsDNA from ssDNA and free nucleotides. Since the dye emits fluorescence only when bound to the target dsDNA, the fluorometric readouts are low in the presence of a polymerase inhibitor.