BRD3 (BD1) Inhibitor Screening Assay Kit

The BRD3 (BD1) Inhibitor Screening Assay Kit is designed to measure the inhibition of BRD3 bromodomain 1 (BD1) binding to its substrate. The BRD3 (BD1) Inhibitor Screening Assay Kit comes in a convenient AlphaLISA® format, with biotinylated histone peptide substrate, assay buffer, detection buffer and purified BRD3 BD1 to perform a total of 384 enzyme reactions. The key to the BRD3 (BD1) Inhibitor Screening Assay Kit is the highly specific binding of the BRD3 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD3 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.