Beadlite(TM) Rapid Colorimetric Amino Quantitation Kit for Nanoparticles

Surface functionalized micro- and nano-particles have been widely used in the field of nanotechnologies and biological sciences such as bio-separation and purification, assay development, and drug delivery. Among the common functional groups, amine group is one of the most popular groups for modification, and is often the first active group to be introduced. Various biomolecules, either probe molecules or target molecules, can thus be immobilized on the surface of nanoparticles through the reaction with these amine groups. The density of amine groups plays a key role in determining the properties of nanomaterials and controlling their interactions with biomolecules. Therefore, quantitation of amine density on the surface of particles is critical to their applications. Beadlite(TM) Rapid Colorimetric Amino Quantitation Kit offers a rapid and sensitive absorption-based assay for measuring amine density with high specificity. Upon the selective reaction with the amine group, the Bucculite(TM) MTA is first immobilized on the surface of particles, and then the modified particles are added to Bucculite(TM) MTA-Dye 650 solution resulting in a decrease of absorbance in supernatant. The signal can be measured by UV-Vis spectrometer at ~650 nm and the change is proportional to the amount of amine groups on the surface of the nanoparticles.