Anti-IgM (Mu Chain) Biotin conjugated

Applications:, Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000. Assayed against 1ug of chicken IgM in a standard capture ELISA using Streptavidin (HRP) (S7973-97A) and ABTS (A0030) as a substrate for 30 minutes at RT. , Western Blot: 1:2000-1:10,000, Immunohistochemistry: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.