Anti-IgG (H&L) X-adsorbed, Peroxidase conjugated

Applications: , Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase electron microscopy and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:4000-1:20,000, 1.0ug of Chicken IgG was used in a standard capture ELISA with ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) A0030 as a substrate for 30 minutes at RT., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.