Anti-PDCD1, CT (Programmed Cell Death 1, PD-1, CD279)

Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC) (1). T-cell activation is generally self-limited as activated T cells express receptors such as PDCD-1 that mediate inhibitory signals from the APC (2). PDCD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PDCD1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens (3). Evidence for this is seen in PDCD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases (4). Despite its predicted molecular weight, PDCD1 often migrates at higher molecular weight in SDS-PAGE. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml. See P3120-61A for control peptide., Immunohistochemistry (paraffin): 2.5ug/ml, Immunofluorescence: 20ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: THP-1 cell lysate, Human brain tissue, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.