Anti-Netrin-1 (NTN1)

Netrin­1 (from Sanskrit netr meaning "to guide", also known as Epididymis tissue protein Li 131P) is a 66­76kD secreted member of the laminin superfamily of molecules. It is expressed by multiple cell types, including embryonic neural tube ventral midline cells plus postnatal renal tubule epithelium and oligodendroglia. Netrin­1 has multiple receptors, including beta 4­integrins, DCC, DSCAM and UNC5A­thru­D. Its effects are context specific. For example, on neurons, binding to either DCC or DSCAM alone promotes chemoattraction, while a DCC:UNC5 complex promotes chemorepulsion. In addition, the ligation of UNC5B on leukocytes suppresses cytokine production, while the binding of netrin to DCC expressed on select cell types inhibits apoptosis. Mature human netrin­1 is 580 amino acids (aa) in length (aa25­604). It contains an N­terminal laminin domain (aa47­284), three EGF­like domains (aa285­453) and a C­terminal cell­interaction netrin­like domain (aa472­601). Over aa22­604, human netrin­1 shares 99% aa sequence identity with mouse netrin­1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.