Anti-Netrin-1 (Epididymis tissue protein Li 131P)

Netrin-1(NTN1)?also known as NTN1L, is a diffusible protein made by floor plate cells. Netrin-1 is found in the floor plate and neuroepithelial cells of the ventral region of the spinal cord, as well as other locations in the nervous system including the somatic mesoderm, pancreas and cardiac muscle. The human NTN1 gene is mapped to chromosome 17p13-p12 by FISH . Netrin-1 can attract spinal commissural axons and repel trochlear axons in vitro. And netrin-1 expression confers a selective advantage for tumor cell survival. The binding of netrin-1 to DCC appears to depend on the presence of a coreceptor or accessory proteins. Adenosine A2b receptor is actually a netrin-1 receptor and induces cAMP accumulation on binding netrin-1, and that netrin-1-dependent outgrowth of dorsal spinal cord axons directly involves A2b. Netrin-1 can promote intestinal tumor development, probably by regulating cell survival. Thus, a netrin-1 receptor or receptors function as conditional tumor suppressors. Expression of Unc5b triggered endothelial cell repulsion in response to netrin-1 in vitro, whereas a truncated Unc5b lacking the intracellular signaling domain failed to induce repulsion. UniProt Number: O95631, Gene ID: NTN1, Applications: Suitable for use in Western Blot. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Handling: Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term., Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.