Anti-Histone H4K20Ac Monoclonal Antibody (RM205)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin. Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription. Acetylation of histone H4 at lysine 20 (H4K20Ac) is associated with gene repression. It is enriched near the transcription start sites of minimally expressed genes and, to a lesser extent, in the gene body regions of highly expressed genes in HeLa-S3 cells. H4K20Ac can be recognized and bound by the bromodomain of the histone acetyltransferase CREB-binding protein (CBP), as well as by the second bromodomain of bromodomain-containing protein 4 (BRD4). Cayman's Histone H4K20Ac Monoclonal Antibody (RM205) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Acetylated Histone H4 Lysine 20. Immunogen: Peptide corresponding to H4K20Ac. Clone Designation: RM205. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H4K20Ac, (-) Unmodified H4K20, (-) H4K5Ac, (-) H4K8Ac, (-) H4K12Ac, (-) H4K16Ac, (-) H4K31Ac, (-) H4K91Ac.