Anti-Granzyme B, Biotin conjugated, clone 3H2181

Granzymes are neutral serine proteases, which are stored in specialized lytic granules of cytotoxic T lymphocytes (CTLs) and in natural killer (NK) cells. A number of granzymes A to G, have been isolated and cloned from mouse CTLs and NK cells, however in man, fewer have been cloned and identified. One of these, human granzyme B, is involved in target cell apoptosis during lymphocyte mediated cytotoxicity. Exocytosis of granzyme containing granules in the cytoplasm of the target cell leads to the induction of DNA fragmentation and apoptosis., Granzyme B is expressed within the granules of cytolytic lymphocytes (CTLs).Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death. Applications: Suitable for use in ELISA. Other applications have not been tested. , Recommended Dilutions: ELISA: 1ug/ml, Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line. Matched Pair: Capture: G8957-15B, Detection: G8957-15E, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.