Anti-Endoplasmic Reticulum Protein 57 (protein disulfide isomerase family A, member 3, ERp57, ERp60,

Alternate Name: protein disulfide isomerase family A, member 3, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, 58 kD microsomal protein, endoplasmic reticulum P58, glucose regulated protein, 58kD, phospholipase C-alpha, protein disulfide isomerase-associated 3, Domain Information: Thioredoxin , Gene Ontology Terms: ER retention, phospholipase C, signal transduction, protein-nucleus import, cysteine-type endopeptidase, protein disulfide isomerase, Application(s): Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 55kD band observed in Human Hepatoblastoma HepG2 lysates (calculated MW of 56.8kD according to NP_005304.3). Recommended concentration: 1-3ug/ml. Staining (1ug/ml) of HepG2 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See E2291-75G-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.