Anti-Chk2 (Checkpoint Kinase 2, Chek 2, Chek2, Chk 2, CDS 1, CDS1, HuCds 1, HuCds1, LFS2, PP1425, R

Checkpoint homolog CHK2 is an important cell cycle checkpoint regulator. The protein is rapidly activated by phosphorylation in response to DNA damage and to replication block. When activated, CHK2 is known to prevent entry into mitosis and has been shown to regulate the tumor suppressor protein p53, leading to cell cycle arrest in G1. At least 12 isoforms are produced by alternative splicing. Applications: Suitable for use in Western Blot. Other applications not tested. , Recommended Dilution: Western Blot: 0.75ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.