Anti-CD42b (Glycoprotein Ib, GPIb), clone 0.N.114

The CD42b glycoprotein, also known as GPIb, is a co-factor of ristocetin-induced aggregation and is involved in the binding of platelets to blood vessel walls. The CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. The absence of CD42b antigen on platelets may indicate Bernard-Soulier disease. CD42b is of value in the immunophenotyping of megakaryoblastic leukemias, particularly when used in conjunction with antibodies to CD41. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. No special pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues., Optimal dilutions to be determined by the researcher. Recommended Positive Control: Tonsil, Placenta, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.