Anti-CD11a (Leukocyte Function Associated Antigen, LFA-1, Integrin aL)

CD11a (also known as integrin alphaL or lymphocyte function-associated antigen (LFA)-1 alpha chain) complex with the Beta2 subunit of the integrin family, CD18, to form the cell surface heterdimer, LFA-1 or CD11a /C18 (alphaLBeta2). LFA-1 is expressed on all leukocytes including lymphocytes, monocytes, and granulocytes. It is involved in leukocyte adhesion to its ligands including intercellular adhesion molecule-1 (ICAM-1 or CD54), ICAM-2 (CD102), ICAM-3 (CD50) and Telencephalin (TLN) and play a role in most immune/inflammatory responses. MAb DF1524 reacted with CD11a transfected cells and potently blocked LFA-1 dependent homotypic cell aggregation. Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:16,000, Western Blot: 0.5-2ug/ml, Immunohistochemistry (Paraffin): 4-6ug/ml. Steamed antigen retrieval with citrate buffer pH 6 is recommended., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.