Anti-Casein Kinase 2 alpha (CKIIa CK2A1, CSNK2A1)

Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer. Applications: Suitable for use in Western Blot, Dot Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 detects Casein Kinase 2 alpha on 10ug of HeLa cell lysates. Also detects CK-2a in extracts of kidney tissue. Various bands are seen between 40 and 44kD. Using 1ug/ml of C2085-63J, the lower detection limit for the holoenzyme is 500ng. The detection limit for the alpha-subunit is 300ng., Dot Blot: 1ug/ml, The lower detection limit for the holoenzyme is 100ng. The detection limit for the alpha-subunit is 60ng., Immunoprecipitation: Immunoprecipitates a protein with a MW of 44kD, CK-2a subunit. A 26kD protein band, the beta-subunit of the heterotetrameric holoenzyme a2b2, is coprecipitated with the CK-2a subunit., Immunohistochemistry: Paraffin sections, Immunocytochemistry (Acetone or methanol fix only): 5ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.