Anti-Akt1 (Rac PKa, PKBa), clone 4H304

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. Applications: Suitable for use in Immunoblot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:5000-1:40,000 detects Akt1/PKBa in RIPA lysates from human HEK293 cells. HEK293 cell lysate or 40ng of purified Akt1 protein, Akt2 protein and Akt3 protein were resolved by electrophoresis, transferred to nitrocellulose and probed with A1125-18 (1:10,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 5ul immunoprecipitated Akt1/PKBa from 500ug of HEK293 RIPA lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.