Sonic Hedgehog, Active (SHH), Recombinant, Human, aa22-197

Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO (By similarity). Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1ug/ml, corresponding to a specific activity of ?1.0x10e3 IU/mg. Amino Acid Sequence: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG, Molecular Weight: ~19.8kD, PubMed ID: 7590746, 12853948, 12690205, 9593755, 7720571, 12837695, 16335952, 18417549, 19847792, 24456159, 24965254, 10753901, 19561609, 20504762, 8896572, 9302262, 10441331, 10556296, 11471164, 11479728, 12503095, 1510e3725, 15107988, 15221788, 15942952, 15942953, 16282375, 17001669, 19603532, Gene Name: SHH, Swiss Prot: Q15465, Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O, BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.