Cell Meter(TM) Intracellular GSH Assay Kit *Optimized for Flow Cytometry*

Cell Meter(TM) Intracellular GSH Assay Kit *Optimized for Flow Cytometry*
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
ABD-22810 1 kit (100 assays) -

3 - 8 Werktage*

230,00 €
 
The Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a... mehr
Produktinformationen "Cell Meter(TM) Intracellular GSH Assay Kit *Optimized for Flow Cytometry*"
The Cell Meter(TM) assay kits are a set of tools for monitoring cell viability. There are a variety of parameters that can be used for monitoring cell viability. This particular kit is designed to monitor cell apoptosis through measuring the depleted antioxidant reduced glutathione (GSH). GSH is involved in many cellular processes including the scavenging of free radicals, drug detoxification, cell signaling, and cell proliferation. The Cell Meter(TM) Intracellular GSH Assay Kit provides all the essential components with an optimized assay method for the detection of apoptosis in cells with the decreased GSH. This fluorometric assay is based on the detection of the GSH in cells using our proprietary non-fluorescent Thiolite Green(TM) dye that becomes strongly fluorescent upon reacting with thiol. In normal cells, Thiolite Green(TM) accumulates primarily in cytosol, but it is partially translocated to mitochondria in apoptotic cells while Thiolite Green(TM) staining intensity decreases. Cells stained with Thiolite Green(TM) can be visualized by flow cytometry with Ex/Em = 490 nm/520 nm (FL1 channel). The kit can be paired with other reagents, such as 7-AAD (Cat# ABD-17501), propidium iodide (Cat# ABD-17517) for multi-parametric study of cell viability and apoptosis. The kit is optimized for screening of apoptosis activators and inhibitors by flow cytometry.
Schlagworte: Glutathione FC Assay
Hersteller: AAT Bioquest
Hersteller-Nr: 22810

Eigenschaften

Anwendung: FC, GSH assays
Format: Homog.

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +20°C (International: +20°C)
Signalwort: Warning
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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