Cell Meter(TM) Fluorimetric Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

The Cell Meter(TM) assay kits are a set of tools for monitoring cell viability and proliferation. There are a variety of parameters that can be used for monitoring cell viability and proliferation. This particular kit is designed to monitor cell cycle progression and proliferation using Nuclear Red(TM) CCS1, a cell cycle stain in fixed cells. The dye passes through a permeabilized membrane and intercalates into cellular DNA. The signal intensity of Nuclear Red(TM) CCS1 is directly proportional to DNA content after RNA is degraded by RNase provided in the kit. The percentage of cells in a given sample that are in G0/G1, S and G2/M phases, as well as the cells in the sub-G1 phase prior to apoptosis can be monitored with a flow cytometer at Ex/Em = 490 nm/620 nm (FL2 channel).