Anti-phospho-Src (Tyr529), clone 32S01

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA. Phospho-Src (Tyr529) antibody detects Src kinase phosphorylated at tyrosine 529, a regulatory modification that maintains Src in an inactive conformation. Src is a non-receptor tyrosine kinase involved in diverse cellular pathways, including proliferation, adhesion, and cytoskeletal dynamics. Phosphorylation at Tyr529 creates an intramolecular interaction that suppresses kinase activity until signaling cues trigger dephosphorylation and activation.Research employing Phospho-Src (Tyr529) antibody has shown that deregulation of this phosphorylation site contributes to oncogenesis. Loss of inhibitory phosphorylation at Tyr529 results in constitutively active Src, promoting tumor cell invasion, angiogenesis, and metastasis. Beyond cancer, Src regulation impacts immune signaling, bone metabolism, and neuronal communication, underscoring its broad biological relevance.Antibodies specific for Src phosphorylated at Tyr529 are used in assays such as western blot, immunoprecipitation, and immunofluorescence. These reagents allow scientists to measure Src activation status and investigate how signaling pathways modulate its phosphorylation. Clone-validated specificity ensures recognition of only the phosphorylated form, avoiding cross-reactivity with total Src protein.NSJ Bioreagents supplies this Phospho-Src (Tyr529) antibody to support research into cancer signaling, kinase regulation, and cellular communication. Alternate names include proto-oncogene tyrosine-protein kinase Src antibody and pp60c-Src antibody.