Anti-phospho-Rac1 (Ser71)

Rac, cdc42 and Rho A, B and C are members of a small RhoGTPase family that bind and hydrolyze GTP. GTP-bound Rac1 and cdc42 play a pivotal role in controlling cell shape, adhesion, growth and transformation. Active Rac1 is implicated in regulating serum response element (SRE), NFAT1 and nuclear factor kB (NF-kB) transcription activities. Activated Rac1 and cdc42 bind and activate PAK1, which in turn activates key downstream signaling proteins including MEKK-1 and JNK. Rac1 and cdc42 are phosphorylated on serine 71, a putative Akt site located between the proteinbinding domain and GTP-binding domain. Phosphorylation of Rac1 on serine 71 regulates its GTP-binding and GTPase activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.