Anti-phospho-cdc2 (Thr15)

Anti-cdc2 pT15 Antibody is directed against rat cdc2 protein phosphorylated ar T15. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated cdc2. Minimal reactivity occurs against non-phosphorylated cdc2. No reactivity occurs after ?-phosphatase treatment. Reactivity against cdc2 occurs from rat, human and xenopus sources. However, reactivity is also expected against the following species based on 100% sequence homology: mouse and zebra fish.