Anti-p53 (Phospho-Ser392)

p53 is a transcription factor and tumor suppressor encoded by TP53 in humans with roles in cell cycle arrest, apoptosis, senescence, differentiation, and DNA repair. It is composed of two N-terminal intrinsically disordered transactivation domains (TADs), a proline-rich domain (PRD), a DNA-binding domain (DBD), a tetramerization domain (TD), and an intrinsically disordered C-terminal domain. p53 is ubiquitously expressed, with protein levels increasing under conditions of extra- or intracellular stress, such as DNA damage, oncogene activation, oxidative stress, or hypoxia. It is activated and translocated to the nucleus in response to cellular stress via post-translational modifications, such as phosphorylation, methylation, and acetylation, where it binds p53 consensus DNA-binding elements and regulates transcription of its target genes in a cell-, tissue-, and stress signal type-specific manner. p53 is phosphorylated at Ser392 by IkappaB kinase beta (IKKbeta) in glutamine-deficient cancer cells. Loss-of-function and/or gain-of-function mutations in TP53 occur in approximately 50% of human cancers. Cayman's p53 (Phospho-Ser392) Polyclonal Antibody can be used for Western blot (WB) applications. This antibody recognizes p53 at ~53 kDa from human and rat samples.Synonyms: Antigen NY-CO-13, Cellular Tumor Antigen p53, Phosphoprotein p53, Transformation-related Protein 53, Tumor Protein p53, Tumor Suppressor p53. Immunogen: Synthetic peptide corresponding to amino acid residues surrounding phospho-Ser392 of human p53 conjugated to keyhole limpet hemocyanin (KLH). MW: ~53 kDa. Formulation: (Request formulation change), 10 µg of affinity-purified IgG polyclonal antibody. Storage Buffer: 100 µl of 10 mM HEPES, pH 7.5, with 50% glycerol, 0.1% BSA, and 150 mM sodium chloride. Host: Rabbit. Isotype: IgG. Applications: WB. Cross Reactivity: (+) Human, (+) Rat p53 (phospho-Ser392). UniProt Accession #: P04637. Origin: Animal/Rabbit.