Anti-Vinculin, clone 0.T.153

Vinculin, a cytoskeletal protein found in adherens type cell-cell and cell-extracellular matrix junctions, displays considerable heterogeneity. At least three 117kD and two 130kD isoforms (muscle specific metavinculin) have been detected. Vinculin binds talin and a-actinin in adhesion plaques and may play either a structural or a regulatory role in these structures. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-1ug/ml detects Vinculin in RIPA lysates of human A431 cells, previous lots detected Vinculin in RIPA lysates from chicken embryonic fibroblasts (CEF) and mouse 3T3 fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with V2122-50 (0.5ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. , Immunoprecipitation: 4ug immunoprecipitates Vinculin from mouse 3T3/A31 and human A431 RIPA lysates. , Immunohistochemistry and Immunocytochemistry (frozen and formalin fixed sections): 10ug/ml will stain Vinculin in Ref-52 cells fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100. , Optimal dilutions to be determined by the researcher. Positive Antigen Control: A0001-50, Non-stimulated A431 cell lysate. Use 20ug per lane for minigels. , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.