Anti-Ubiquilin 1 (DA41, DSK2, PLIC-1, UBQLN1, UBQN, XDRP1, PLIC-1, Protein Linking IAP with Cytoskel

Ubiquilin­1 (also known as DA41 and PLIC­1/Protein Linking IAP with Cytoskeleton 1) is a 62­70kD intracellular member of the UBL­UBA family of proteins. Consistent with its name, it ubiquitously expressed, and is particularly prominent in skeletal muscle and neurons. Multiple functions have been attributed to ubiquilin­1. For instance, it is now known to be a key player in APP processing. On the cleavage side, ubiquilin­1 binds to PS/presenilin, a component of the gamma­secretase complex that cleaves APP. Ubiquilin­1 appears to target both ubiquitinated single chain PS to the proteosome, and aggregated PS to the aggresome. With respect to APP, ubiquilin­1 serves as a molecular chaperone, binding to and inhibiting APP aggregation. On cells expressing CD47, Ubiquilin­1 acts as an intermediary between integrins, the cytoskeleton and CD47, promoting cell spreading. And in neurons, ubiquilin­1 stabilizes both membrane and GABA subunit stability, enhancing inhibitory synaptic transmission. Human ubiquilin­1 is 589 amino acids (aa) in length. It contains one ubiquitin­like domain (aa37­111), an S5a binding site (aa72­105) and a UBA binding region (aa548­585). There are multiple splice variants, ranging from 19­59kD in size. One shows a deletion of aa418­445, a second contains a three aa substitution for aa151­589, a third possesses a 29aa substitution for aa112­589, and a fourth shows a deletion of aa61­237 coupled to a second deletion of aa361­589. Over aa546­585, human and mouse ubiquilin­1 are identical in amino acid sequence. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.