Anti-tam

Make to order. Production time: 70-80 business days. Deliverables: 1. 200 µg antigen (used as positive control), 2. 1 ml pre-immune serum (used as negative control) , 3. Rabbit polyclonal antibody, antigen affinity purified. Quality Guarantee: 1. Antibody purity > 90% confirmed by SDS-PAGE, 2. Antibody titer > 1: 64,000 confirmed by ELISA, 3. WB positive with the immunogen protein (not applicable for synthetic peptide). Applications: ELISA, WB (positive for the recombinant immunogen protein/peptide). Buffer: Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. Protein function: As the catalytic component of the DNA polymerase gamma complex is involved in the replication of mitochondrial DNA (mtDNA) (PubMed:11917141, PubMed:15537632, PubMed:19924234, PubMed:28430993, PubMed:3095323, PubMed:7499423, PubMed:8798543). Has both 5'-3' DNA polymerase and a highly mispair-specific 3'-5' exonuclease activity (PubMed:15537632, PubMed:26554610, PubMed:2671990, PubMed:28430993, PubMed:7499423, PubMed:8798543). At the end of mtDNA replication DNA ends are ligated to produce a closed circular mtDNA molecule, its exonuclease activity is required for formation of these ligatable ends by preventing DNA synthesis from continuing past the 5'-end of downstream DNA into duplex DNA regions (PubMed:28430993). Does not possess DNA primase activity, does not catalyze strand displacement synthesis and does not contain a 5'-3' exonuclease activity to catalyze nick translation (PubMed:3095323). Important for promoting the elimination of paternal mitochondrial DNA during spermatogenesis, however its exact role in this function has not yet been identified and appears to be independent of its 3'-5'-exonuclease activity and only partially dependent on its DNA polymerase activity (PubMed:28318978). [The UniProt Consortium]