Anti-Surfactant Protein A (SP-A)

There are four main surfactant proteins, known as SP-A, B, C, and D. SP-A was the first surfactant protein to be identified, and is also the most abundant. Its molecular mass varies from 26-38kD. It can be found in an open or closed form depending on the other substances present in the system. Calcium ions produce the closed-bouquet form. SP-A is necessary for the production of tubular myelin, a lipid transport structure unique to the lungs. Applications: Suitable for use in Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: 1:200, Western Blot: 0.5ug/ml. Detected SP-A in 10ug of human lung tissue lysate, band at ~35kD., Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x Rb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x Rb, I1904-46Q: IgG, H&L (HRP) Pab Gt x Rb, I1904-43: IgG, X-Adsorbed (Biotin) Pab Gt x Rb, I1904-40E: IgG, H&L (Biotin) Pab Gt x Rb, I1904-46P: IgG, H&L (Biotin) Pab Gt x Rb, I1904-42T: IgG, H&L (Biotin) Pab Gt x Rb, I1904-42V: IgG, H&L (Biotin) Pab Gt x Rb, Recommended Control: Human lung tissue lysate, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.