Anti-Slamf6 (Ly108, SLAM Family Member 6, CD352, NK-T-B-Antigen, NTB-A, Lymphocyte Antigen 108)

SLAM family member 6 (UniProt Q9ET39, also known as CD352, NK-T-B-antigen, NTB-A, Lymphocyte antigen 108) is encoded by the Slamf6 (also known as Ly108) gene (Gene ID 30925) in murine species. Ly108/Slamf6 belongs to the 7-member (SLAM/CD150, CD48/SLAMF2, Ly-9/CD229, 2B4/CD244, CD84/SLAMF5, Ly108/NTB-A/CD352, CRACC) signaling lymphocytic activation molecule family (SLAMF) of receptors broadly expressed in hematopoietic cells. 2B4 and CD48 interact with eath other, while rest of the family members act as self-ligands, where two identical molecules on the surface of two different cells can interact as receptor and ligand to each other. SLAMF members carry one or more copies of immunoreceptor tyrosine-based switch motif or ITSM (TxYxxV/I) in their cytoplasmic tails. Upon phosphorylation on the tyrosine residue, ITSM mediates the recruitment of SH2 domain-containing signaling molecules such as SLAM-associated protein (SAP), including SAP in T-, NK, and NK T-cells, EAT-2A and EAT-2B (murine) in NK cells and APCs. Ly108/Slamf6 is synthesized with a signal peptide sequence (mouse aa1-30), the removal of which yields the mature Ly108 with an extracellular region (aa31-239) that contains a V-type and a C2-type Ig-like domain (aa36-130 and 147-210), followed by a single transmembrane domain (aa240-262) and a cytoplasmic tail (aa263-351). Ly108/Slamf6 cytoplasmic domain contains two copies of ITSM (mouse aa293-TVYAQV-298 and 317-TIYSIV-322). Alternative splicings produce three Ly108 isoforms, Ly108-1 (Ly108s, UniProt Q9ET39-2), Ly108-2 (Ly108l, UniProt Q9ET39-1) and Ly108-3 (Not reported by UniProt), that differ in their cytoplasmic C-terminal end sequence, but are identical in the rest of their sequence. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Function Assay. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug of 1x10e6 mouse splenocytes cells, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.