Anti-Sialoprotein, Bone (BSP, Bone sialoprotein 2, BSP2, Cell-binding sialoprotein, Integrin-binding

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, RIA and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Paraffin): 1:400-1:2000, Immunohistochemistry (Frozen): 1:100, Immunocytochemistry: 1:200, Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.