Anti-Sialoprotein, Bone (BSP, Bone sialoprotein 2, BSP2, Cell-binding sialoprotein, Integrin-binding

Bone sialoprotein (BSP) is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70-80kD. The protein has been found in osteoblasts and osteocytes. BSP is synthesized and secreted by bone-, dentin- and cementum-forming cells. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. Applications: Suitable for use in ELISA, Western Blotting, RIA, Immunocytochemistry, Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml, Western Blotting: 2ng/ml, Immunohistochemistry: 1:100, paraffin sections (2 g/ml),, RIA: 20ng/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.