Anti-PLD2 (Phospholipase D2, PLD 2, hPLD2, EC=3.1.4.4, Choline Phosphatase 2, PLD1C, Phosphatidylcho

PLD2 is a peripheral membrane protein belonging to phospholipase D family with a PH domain, two PLD phosphodiesterase domains and a PX (phox homology) domain that plays a regulatory rather than catalytic role. PLD2 catalyzes the hydrolysis of phosphatidylcholine (PC) to produce phosphatidic acid and choline. PLD2 may function in regulated secretion, signal-induced cytoskeletal regulation and/or endocytosis, transcriptional regulation, and cell cycle control. It is usually stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and activated by the ADP-ribosylation factor-1 (ARF-1). PLD2 is activated by agonist stimulation of both tyrosine kinase and G protein-coupled receptors and is also known to interact with EGFR and PIP5K1A. PLD2, localized to plasma membrane caveolae, is ubiquitously expressed in most tissues. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (formalin fixed paraffin embedded): 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.