Anti-PARP 1, cleaved (Asp214) (Poly ADP-Ribose Polymerase 1, Poly [ADP-ribose] Polymerase 1, PARP1,

PARP, a 116kD nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24kD) from the carboxy-terminal catalytic domain (89kD) (2,4). PARP helps cells to maintain their viability, cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6). Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:400, Flow Cytometry: 1:400, Western Blot: 1:1000, Immunoprecipitation: 1:100, Immunohistochemistry (paraffin): 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.