Anti-NRAS (Neuroblastoma RAS Viral (v-ras) Oncogene Homolog, N-Ras, ALPS4, AV095280, GTPase NRas, HR

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated to a GTP-bound form by a GTPase activating protein and inactivated to a GDP-bound form by a guanine nucleotide-exchange factor. Defects in this gene are a cause of juvenile myelomonocytic leukemia (JMML). Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other Applications not tested. Recommended Dilutions: ELISA: 1:32,000, Western Blot: 0.01-0.03ug/ml, Immunohistochemistry (Paraffin): 2-3ug/ml Requires HEIR using citrate buffer pH6.0, Optimal dilutions to be determined by the researcher. Positive Control: Human lysates of cell line A431, Blocking Peptide: N5385-01A1, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 monthsat -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.