Anti-Lipocalin 2 (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil g

Lipocalins are a widespread family of small, robust proteins that typically transport or store biological compounds which are either of low solubility or are chemically sensitive, including vitamins, steroids, odorants and metabolic products.The members of this lipocalins share a high similarity in their tertiary structures in spite of a low degree in amino-acid sequence identity: three highly conserved sequence motifs form a funnel-like beta barrel which encloses a hydrophobic pocket for the internal ligand binding. The human neutrophil lipocalin (HNL) is produced by neutrophil leukocytes and stored in intracellular specific granules. Neutrophil lipocalin is covalently linked to gelatinase B (MMP-9) and proMMP is stored in three forms: the monomeric form (92kD), the homodimer (220kD) and a heterodimer (125kD). During inflammation and neoplastic transformation the human neutrophil lipocalin (HNL) is produced in neutrophils and in epithelial cells. Furthermore neutrophil lipocalin is a superior means to distinguish acute bacterial and viral infections as well as it enables the determination of the neutrophil activation level. Applications: Suitable for use in Immunoassays and Immunofluorescence. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:50, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.