Anti-Fibronectin, clone 5F124

Fibronectin seems to play a key role in the tumor cell extracellular matrix during invasive tumor growth. Increasing expression of stromal fibronectin has been reported during tumor progression. Applications: Suitable for use in Immunoblot and Immunohistochemistry (frozen tissue and cell preparations, on paraffin-embedded tissue after microwave treatment), Recommended Dilution: Immunohistochemistry: 1:5-1:10, Incubation Time 1 hr at room temperature. Positive Control: Human skin, ulcerating tissue. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.