Anti-ERBB2 (HER-2, CD340)

This monoclonal antibody is specific to c-erb B-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erb B-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. Applications: Suitable for use in ELISA, Flow Cytometry and Immunofluorescence. Other applications not tested. Recommended Dilutions: ELISA: Coating, Flow Cytometry: 1.2ug/10e6 cells, Immunofluorescence: 1-4ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: MCF-7, SKBR-3 Cells or Breast Cancers, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.