Anti-EDG1 (Endothelial Cell Differentiation Gene 1) (PE)

EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells. Validated using CHO cell line transfected with EDG1, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. Add 10ul of E2208-01E to <1x10e6 cells and incubate for 30 minutes at RT., 3. Removed unbound antibody by washing the cells twice with Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point. , 4. Resuspend the cells in F4568-10 for analysis. As a control, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.