Anti-Dnmt3b (DNA Methyltransferase 3B, ICF1, DNA MTase HsaIIIB)

DNMT3B (DNA methyltransferase 3B, also DNA MTase HsaIIIB) is a 95-110kD member of the C5-methyltransferase family of enzymes. It is ubiquitously expressed in the embryo, and found associated with nuclear pericentric heterochromatin. Pericentric heterochromatin is chromatin composed of satellite repeats that lie adjacent to the centromere. DNA (cytosine-guanine dinucleotides) associated with this area undergos methylation, contributing to gene repression and proper chromosome segregation. Human DNMT3B is 853 amino acids (aa) in length. It contains a PWWP (Pro-Trp-Trp-Pro) domain that binds DNA (aa225-283), a ADD (ATRX, DNMT3, DNMT3L) domain that contains a Zn-finger and binds to an unmethylated histone H3 tail (aa423-555), and a class I AdoMet-MTase catalytic region that uses S-adenosyl-L-homocysteine as a substrate for methyl transfer (aa577-847). There are at least five isoform variants. All show a deletion of aa365-375. In addition, a second contains an alternative start site 12aa upstream of the standard site, a third shows a deletion of aa745-807, a fourth contains a Ser substitution for aa744-853, while a fifth possesses a 45aa substitution for aa768-853. Over aa1-355, human DNMT3B shares 73% aa sequence identity with mouse DNMT3B. Applications: Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.2ug/ml, Immunocytochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.