Anti-CD83

Human CD83 is a type 1 cell surface glycoprotein expressed almost solely by dendritic cells that play an important role in antigen presentation (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ug/ml, 80ul labels 5x10e5 Raji cells, Immunohistochemistry: frozen sections, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured Raji cells per tube were incubated 45 minutes on ice with 80ul of C2435-04 at 10ug/ml. , 2. Cells were washed twice and incubated with 2º reagent Goat anti-Mouse IgG/FITC., 3. Cells were then washed three times, fixed and analyzed by FACS. , 4. Cells stained positive with a mean shift of 0.66 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Recommended Secondary Antibodies: I1904-04A: IgG, H&L (FITC) Pab Gt xMo, I1904-06F: IgG, H&L (FITC) (X-absorbed) Pab Gt xMo, I1904-14A: IgG, Fc (FITC) Pab Gt xMo, I1904-25A: IgG H&L (FITC) Pab Gt xMo, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.