Anti-CD59 (MACIF, MIRL, Protectin), FITC conjugated, clone 2Q1428

CD59 is identical to other CD59 monoclonals, MEM-43 and YTH 53.1, defined at the 4th International Conference on Human Leukocyte Differentiation Antigens(4,5). CD59 is an inhibitor of attack by autologous complement(6) possibly by limiting incorporation of C9 into the membrane complex(7,8). GPI linked proteins such as CD59 are deficient on cells from patients with the acquired clonal disorder paroxysmal nocturnal haemoglobulinuria (PNH) in which the erythrocytes are susceptible to lysis by complement and leading to anemia(9). Evidence suggests that CD59 may be the most important complement regulatory protein missing in PNH(1,10). Applications: Studies of complement and homologous restriction of complement action of the properties of GPI linked proteins and in the characterisation of cells from patients with PNH. , Recommended Dilution: , Flow cytometry and indirect immunofluorescence: 1:25. Dilute with isotonic buffer. Use 50ul of the appropriate dilution per 1x10e6 cells in 100ul buffer. , Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. , Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.