Anti-CD59 (MACIF, MIRL, Protectin), clone 2Q1428

Identical to other CD59 monoclonals, MEM-43 and YTH 53.1, defined at the 4th International Conference on Human Leukocyte Differentiation Antigens. CD59 is an inhibitor of attack by autologous complement possibly by limiting incorporation of C9 into the membrane complex. GPI linked proteins such as CD59 are deficient on cells from patients with the acquired clonal disorder paroxysmal nocturnal haemoglobulinuria (PNH) in which the erythrocytes are susceptible to lysis by complement and leading to anemia. Evidence suggests that CD59 may be the most important complement regulatory protein missing in PNH. Applications: Suitable for use in Flow Cytometry, Indirect Immunofluorescence, and ELISA. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:25, Indirect Immunofluorescence: 1:25 Dilute with isotonic buffer. Use 50ul of the appropriate dilution per 1x10e6 cells in 100ul buffer. , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , , Warning: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.