Anti-CD5 (Lyt-1), Phycoerythrin conjugated, clone 5K6

CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors. Applications: Suitable for use in Flow Cytometry for the enumeration and identification of CD5+ cells, and in Immunohistochemistry (frozen sections), Western Blot, and T-cell activation. Other applications not tested. Recommended Dilution: Flow Cytometry: < 0.2ug/10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Store at 2-8°C. Do Not Freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. PE conjugates are sensitive to light.