Anti-CD49f (CD49 antigen-like family member F, CD49f, DKFZp686J01244, FLJ18737, Integrin alpha-6, IT

Human CD49f molecules are integrin alpha-6 chains that are expressed as heterodimers with CD29 (beta1 integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured Raji human tumor cells were washed and incubated 45 min on ice with 80ml of anti-CD49f antibody at 10mg/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.