Anti-Catenin, beta, clone 2Q238

The transmembrane, calcium dependent molecule E-cadherin is considered to be one of the key molecules in the formation of the intercellular junctional complex and establishment of polarity in epithelial cells. Catenins, the alpha-subunit (102kD), beta-subunit (88kD) and gamma-subunit (82kD), are a group of proteins that interact with the intercellular domain of E-cadherin, resulting in complexes of E-cadherin/beta-catenin/alpha-catenin or E-cadherin/gamma-catenin/alpha-Catenin. Beta-catenin, the vertebrate homologue of Drosophila segment polarity gene armadillo, and key element of the Wnt signal transduction pathways, is important in embryogenesis, cell transformation and intercellular adhesion including the adhesion, motility and metastasis of cancer cells. Thus, catenin protein expression, regulation and localization are potentially important markers to predict motility and invasiveness of epithelial neoplasms. The regions of both alpha- and beta-catenin, located on 5q21-22 and 3p21, have been shown to be involved in the development of certain tumors and reduced expressions of both alpha- and beta-catenin have been described in various tumors including breast carcinoma. Besides adhesion functions, beta-catenin binds to the tumor suppressor gene APC. APC mutation disturbs the equilibrium and cytoplasmic levels of free beta-catenin level in the cell and may have a role in tumorigenesis. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Immunohistochemistry: Histochoice fixative is effective. , Immunocytochemistry: Effective for A431 and HT1080 cell lines., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.